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Rat Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit

Rat Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit

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Specification

Product name Rat Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT18 in samples. An antibody specific for KRT18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KRT18; CYK18; K18; cell proliferation-inducing protein 46; cytokeratin 18

Product Properties

Kit components • Rat Keratin, type I cytoskeletal 18 microplate
• Rat Keratin, type I cytoskeletal 18 standard
• Rat Keratin, type I cytoskeletal 18 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KRT18. No significant cross-reactivity or interference between Rat KRT18 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Human keratin 18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Endo B and its coexpressed type II keratin, Endo A (the mouse equivalent of human keratin 8), appear to be the first IF proteins expressed during murine development.
Gene ID 294853
Alternative KRT18; CYK18; K18; cell proliferation-inducing protein 46; cytokeratin 18
Accession Q5BJY9

Image & description

Fig.1. Rat Keratin, type I cytoskeletal 18 (KRT18) Standard Curve.

Fig.1. Rat Keratin, type I cytoskeletal 18 (KRT18) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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