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Rat Intestinal-type alkaline phosphatase (ALPI) ELISA Kit

Rat Intestinal-type alkaline phosphatase (ALPI) ELISA Kit

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Specification

Product name Rat Intestinal-type alkaline phosphatase (ALPI) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Intestinal-type alkaline phosphatase (ALPI) ELISA Kit employs a two-site sandwich ELISA to quantitate ALPI in samples. An antibody specific for ALPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALPI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ALPI; IAP; Kasahara isozyme; alkaline phosphomonoesterase; glycerophosphatase; intestinal alkaline phosphatase

Product Properties

Kit components • Rat Intestinal-type alkaline phosphatase microplate
• Rat Intestinal-type alkaline phosphatase standard
• Rat Intestinal-type alkaline phosphatase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Intestinal-type alkaline phosphatase (ALPI) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ALPI. No significant cross-reactivity or interference between Rat ALPI and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase.The deduced polypeptide showed 86.5% amino acid identity to placental ALP and 56.6% amino acid identity to liver/bone/kidney ALP. Thus, the immunologic cross-reactivity of ALPI and ALPP is perhaps explained. Calf Intestinal Alkaline Phosphatase (CIP) is an enzyme that catalyzes the 5' phosphate group's removal from DNA. This enzyme is frequently used in DNA sub-cloning, because DNA fragments which lack the 5' phosphoryl termini cannot self-ligate. Preventing self ligation is important both in improving the yield of properly ligated product, and reducing the background of improperly self-ligated contaminant.
Alternative ALPI; IAP; Kasahara isozyme; alkaline phosphomonoesterase; glycerophosphatase; intestinal alkaline phosphatase
Accession P15693

Image & description

Fig.1. Rat Intestinal-type alkaline phosphatase (ALPI) Standard Curve.

Fig.1. Rat Intestinal-type alkaline phosphatase (ALPI) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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