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Rat Interleukin-17F (IL17F) ELISA Kit

Rat Interleukin-17F (IL17F) ELISA Kit

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Product name Rat Interleukin-17F (IL17F) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Interleukin-17F (IL17F) ELISA Kit employs a two-site sandwich ELISA to quantitate IL17F in samples. An antibody specific for IL17F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL17F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL17F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL17F bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative IL17F; IL-17F; ML-1; ML1; cytokine ML-1

Product Properties

Kit components • Rat Interleukin-17F microplate
• Rat Interleukin-17F standard
• Rat Interleukin-17F detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Interleukin-17F (IL17F) ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL17F. No significant cross-reactivity or interference between Rat IL17F and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL-17F has been shown to stimulate prolifertion and activation of T cells and PBMCs, to inhibit angiogenesis as well as to regulate cartilage matrix turnover. This recombinant IL-17F is a homodimeric protein with a total weight of 30.1kDa containing two 134 amino acid residue chains.Using nested RACE PCR with leukocyte-anchored cDNA, Starnes et al. (2001) isolated an IL17F cDNA encoding a 153-amino acid protein that shares 40% homology with IL17. In addition to the 2 invariant disulfide bonds found in other members of the IL17 family, IL17F contains a signal peptide with an extra cys-cys pair that could function as an extra disulfide bond. Expression of IL17F could not be identified by Northern blot analysis. RT-PCR analysis detected expression in activated, but not resting, CD4+ T cells and activated monocytes.
Gene ID 301291
Alternative IL17F; IL-17F; ML-1; ML1; cytokine ML-1
Accession Q5BJ95

Image & description

Fig.1. Rat Interleukin-17F (IL17F) Standard Curve.

Fig.1. Rat Interleukin-17F (IL17F) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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