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Rat Heparin-binding EGF-like growth factor (HBEGF) ELISA Kit

Rat Heparin-binding EGF-like growth factor (HBEGF) ELISA Kit

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Specification

Product name Rat Heparin-binding EGF-like growth factor (HBEGF) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Heparin-binding EGF-like growth factor (HBEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate HBEGF in samples. An antibody specific for HBEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHBEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HBEGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HBEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative HBEGF; DTR; DTS; DTSF; HEGFL; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); heparin-binding epide

Product Properties

Kit components • Rat Heparin-binding EGF-like growth factor microplate
• Rat Heparin-binding EGF-like growth factor standard
• Rat Heparin-binding EGF-like growth factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Heparin-binding EGF-like growth factor (HBEGF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat HBEGF. No significant cross-reactivity or interference between Rat HBEGF and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. Diphtheria toxin inhibits protein synthesis in eukaryotic cells by catalyzing inactivation of elongation factor-2 (EF2). Entry of toxin into cells is dependent on binding to the cell surface. Mice and rats are resistant to diphtheria toxin, and the resistance extends to cultured cells. Mouse-human hybrid cells are sensitive to diphtheria toxin. Creagan et al. (1975) showed that sensitivity is determined by human chromosome 5, which presumably carries a gene for a receptor for toxin.
Gene ID 25433
Alternative HBEGF; DTR; DTS; DTSF; HEGFL; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); heparin-binding epide
Accession Q06175

Image & description

Fig.1. Rat Heparin-binding EGF-like growth factor (HBEGF) Standard Curve.

Fig.1. Rat Heparin-binding EGF-like growth factor (HBEGF) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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