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Rat Heart- and neural crest derivatives-expressed protein 1 (HAND1) ELISA Kit

Rat Heart- and neural crest derivatives-expressed protein 1 (HAND1) ELISA Kit

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Specification

Product name Rat Heart- and neural crest derivatives-expressed protein 1 (HAND1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Heart- and neural crest derivatives-expressed protein 1 (HAND1) ELISA Kit employs a two-site sandwich ELISA to quantitate HAND1 in samples. An antibody specific for HAND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHAND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative HAND1; Hxt; Thing1; bHLHa27; eHand; basic helix-loop-helix transcription factor HAND1

Product Properties

Kit components • Rat Heart- and neural crest derivatives-expressed protein 1 microplate
• Rat Heart- and neural crest derivatives-expressed protein 1 standard
• Rat Heart- and neural crest derivatives-expressed protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Heart- and neural crest derivatives-expressed protein 1 (HAND1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat HAND1. No significant cross-reactivity or interference between Rat HAND1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Heart- and neural crest derivatives-expressed protein 1 is protein belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.Plays an essential role in early trophoblast differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes.
Alternative HAND1; Hxt; Thing1; bHLHa27; eHand; basic helix-loop-helix transcription factor HAND1
Accession P57100

Image & description

Fig.1. Rat Heart- and neural crest derivatives-expressed protein 1 (HAND1) Standard Curve.

Fig.1. Rat Heart- and neural crest derivatives-expressed protein 1 (HAND1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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