|Product name||Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit|
|Applications notes||This Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit employs a two-site sandwich ELISA to quantitate GHRP-Ghrelin in samples. An antibody specific for GHRP-Ghrelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGHRP-Ghrelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRP-Ghrelin is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRP-Ghrelin bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Growth hormone releasing peptide-Ghrelin microplate
• Rat Growth hormone releasing peptide-Ghrelin standard
• Rat Growth hormone releasing peptide-Ghrelin detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GHRP-Ghrelin. No significant cross-reactivity or interference between Rat GHRP-Ghrelin and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Growth hormone secretagogue receptor is a G protein-coupled receptor which binds ghrelin and plays a role in energy homeostasis and regulation of body weight.Ghrelin is an appetite-regulating factor secreted from peripheral organs which is involved in regulation of energy homoeostasis via binding to the receptor resulting in the secretion of growth hormone by the pituitary gland. The pathway activated by binding of Ghrelin to the Growth hormone secretagogue receptor, GHSR1a, regulates the activation of the downstream mitogen-activated protein kinase, Akt, nitric oxide synthase, and AMPK cascades in different cellular systems. One of the important features of GHSR1a displays constitutive activity possessing basal activity in the absence of an agonist, resulting in a high degree of receptor internalization as well as of signaling activity.|
Fig.1. Rat Growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.