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Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit

Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit

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Specification

Product name Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA2G10 in samples. An antibody specific for PLA2G10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PLA2G10; GXPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367; SPLA2;

Product Properties

Kit components • Rat Group 10 secretory phospholipase A2 microplate
• Rat Group 10 secretory phospholipase A2 standard
• Rat Group 10 secretory phospholipase A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLA2G10. No significant cross-reactivity or interference between Rat PLA2G10 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The A2 phospholipases (PLA2s; EC 3.1.1.4) catalyze the release of fatty acids from phospholipids and play a role in a wide range of physiologic functions. Cupillard et al. (1997) cloned a novel secretory PLA2, termed GXSPLA2, from a human fetal lung cDNA library. The sequence encodes a 165-amino acid polypeptide, including the 42-amino acid signal peptide. Northern blot analysis revealed that GXSPLA2 expresses an 0.8-kb mRNA in pancreas, lung, and colon and a 1.5-kb mRNA in spleen, thymus, and peripheral blood leukocytes. Expression studies showed that the cDNA encodes an active phospholipase which was secreted into the culture medium.Cupillard et al. (1997) used in situ hybridization to map the PLA2G10 gene to human chromosome 16p13.1-p12.
Gene ID 29359
Alternative PLA2G10; GXPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367; SPLA2;
Accession Q9QZT3

Image & description

Fig.1. Rat Group 10 secretory phospholipase A2 (PLA2G10) Standard Curve.

Fig.1. Rat Group 10 secretory phospholipase A2 (PLA2G10) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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