|Product name||Rat Glutamic acid decarboxylase autoantibody IgM (GAD-Ab-IgM) ELISA Kit|
|Applications notes||This Rat Glutamic acid decarboxylase autoantibody IgM (GAD-Ab-IgM) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAD-Ab-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated GAD-Ab-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled GAD-Ab-IgM and unlabeled GAD-Ab-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GAD-Ab-IgM in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Glutamic acid decarboxylase autoantibody IgM microplate
• Rat Glutamic acid decarboxylase autoantibody IgM standard
• Rat Glutamic acid decarboxylase autoantibody IgM detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Glutamic acid decarboxylase autoantibody IgM (GAD-Ab-IgM) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GAD-Ab-IgM. No significant cross-reactivity or interference between Rat GAD-Ab-IgM and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Glutamate decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms encoded by two different genes - GAD1 and GAD2. These isoforms are GAD67 and GAD66 wit检ʰ桰ʰ|
Fig.1. Rat Glutamic acid decarboxylase autoantibody IgM (GAD-Ab-IgM) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.