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Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) ELISA Kit

Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) ELISA Kit

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Specification

Product name Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAD-Ab-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated GAD-Ab-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled GAD-Ab-IgG and unlabeled GAD-Ab-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GAD-Ab-IgG in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative GAD-Ab-IgG

Product Properties

Kit components • Rat Glutamic acid decarboxylase autoantibody IgG microplate
• Rat Glutamic acid decarboxylase autoantibody IgG standard
• Rat Glutamic acid decarboxylase autoantibody IgG detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GAD-Ab-IgG. No significant cross-reactivity or interference between Rat GAD-Ab-IgG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glutamate decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms encoded by two different genes - GAD1 and GAD2. These isoforms are GAD67 and GAD65 with molecular weights of 67 and 65 kDa, respectively. GAD1 and GAD2 are expressed in the brain where GABA is used as a neurotransmitter, GAD2 is also expressed in the pancreas. At least two more forms, GAD25 and GAD44 (embryonic; EGAD) are described in the developing brain. They are coded by the alternative transcripts of GAD1, I-80 and I-86: GAD25 is coded by both, GAD44 - only by I-80. GAD65 and GAD67 synthesize GABA at different locations in the cell, at different developmental times, and for functionally different purposes. GAD67 is spread evenly throughout the cell while GAD65 is localized to nerve terminals.
Alternative GAD-Ab-IgG

Image & description

Fig.1. Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) Standard Curve.

Fig.1. Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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