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Rat Gastricsin (PGC) ELISA Kit

Rat Gastricsin (PGC) ELISA Kit

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Product name Rat Gastricsin (PGC) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Gastricsin (PGC) ELISA Kit employs a two-site sandwich ELISA to quantitate PGC in samples. An antibody specific for PGC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PGC; RP11-298J23.1; FLJ99563; PEPC; PGII; gastricsin; pepsin C; pepsinogen group; preprogastricsin

Product Properties

Kit components • Rat Gastricsin microplate
• Rat Gastricsin standard
• Rat Gastricsin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Gastricsin (PGC) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PGC. No significant cross-reactivity or interference between Rat PGC and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pepsinogen, the inactive precursor of pepsin belongs to the group of aspartic proteinases, is synthesized in the chief cells of gastric glands. Seven electrophoretically separable pepsinogens exist in human gastric mucosa. One group, called PGA or group I pepsinogen, is characterized by electrophoretically faster migration and localization in the fundus and body of the stomach; the second group, termed pepsinogen C, is localized in the whole stomach. PGCs are found in prostate and in seminal fluid whereas PGAs are not. the absence of detectable immunologic crossreactivity between the pepsinogens A and C results from divergent evolution of sequences located on the surface of the zymogens in contrast to the strongly conserved active site regions located within the binding cleft of the enzymes, inaccessible for antigenic recognition.
Gene ID 100009323
Alternative PGC; RP11-298J23.1; FLJ99563; PEPC; PGII; gastricsin; pepsin C; pepsinogen group; preprogastricsin
Accession Q9GMY2

Image & description

Fig.1. Rat Gastricsin (PGC) Standard Curve.

Fig.1. Rat Gastricsin (PGC) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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