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Rat Galectin-4 (LGALS4) ELISA Kit

Rat Galectin-4 (LGALS4) ELISA Kit

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Product name Rat Galectin-4 (LGALS4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Galectin-4 (LGALS4) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS4 in samples. An antibody specific for LGALS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LGALS4; GAL4; L36LBP; L-36 lactose-binding protein; galectin 4; galectin-4; lactose-binding lectin 4

Product Properties

Kit components • Rat Galectin-4 microplate
• Rat Galectin-4 standard
• Rat Galectin-4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Galectin-4 (LGALS4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LGALS4. No significant cross-reactivity or interference between Rat LGALS4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The two domains have all the conserved galectin signature amino acids, but their overall sequences are only approximately 40% identical. Both domains bind lactose with a similar affinity as other galectins, but their respective preferences for other disaccharides, and larger saccharides, are distinctly different. Thus galectin-4 has a property of a natural cross-linker, but in a modified sense since each domain prefers a different subset of ligands. Similarly to other galectins, galectin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine.
Gene ID 25474
Alternative LGALS4; GAL4; L36LBP; L-36 lactose-binding protein; galectin 4; galectin-4; lactose-binding lectin 4
Accession P38552

Image & description

Fig.1. Rat Galectin-4 (LGALS4) Standard Curve.

Fig.1. Rat Galectin-4 (LGALS4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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