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Rat Galectin-1 (LGALS1) ELISA Kit

Rat Galectin-1 (LGALS1) ELISA Kit

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Specification

Product name Rat Galectin-1 (LGALS1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Galectin-1 (LGALS1) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS1 in samples. An antibody specific for LGALS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LGALS1; DKFZp686E23103; GAL1; GBP; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; galaptin; galectin 1; galectin-1; lactose-binding lectin 1

Product Properties

Kit components • Rat Galectin-1 microplate
• Rat Galectin-1 standard
• Rat Galectin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Galectin-1 (LGALS1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LGALS1. No significant cross-reactivity or interference between Rat LGALS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Galectin-1 is one of the most important lectins to date participating in the malignant tumor development. With more and more data available on its expression in tumors it is already clear that this lectin is an important target for cancer diagnostics and treatment.Its first involvement in cancer was associated with cell adhesion. Later on the lectin was shown to induce apoptosis of activated T-cells, and experiments have confirmed that cancer cells express galectin-1 also to protect themselves from immune response. Latest finding is a participation of galectin-1 in tumor angiogenesis as an angiogenic factor. In most of the cases described, galectin-2 is up-regulated in tumors in comparison to normal tissue. Moreover, the presence of the lectin is associated with metastases and poor prognosis of disease outcome.
Gene ID 56646
Alternative LGALS1; DKFZp686E23103; GAL1; GBP; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; galaptin; galectin 1; galectin-1; lactose-binding lectin 1
Accession P11762

Image & description

Fig.1. Rat Galectin-1 (LGALS1) Standard Curve.

Fig.1. Rat Galectin-1 (LGALS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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