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Rat Fibrinogen alpha chain (FGA) ELISA Kit

Rat Fibrinogen alpha chain (FGA) ELISA Kit

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Specification

Product name Rat Fibrinogen alpha chain (FGA) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Fibrinogen alpha chain (FGA) ELISA Kit employs a two-site sandwich ELISA to quantitate FGA in samples. An antibody specific for FGA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FGA; Fib2; MGC119422; MGC119423; MGC119425; fibrinogen; A alpha polypeptide; fibrinogen; alpha chain; isoform alpha preproprotein; fibrinogen; alpha polypeptide

Product Properties

Kit components • Rat Fibrinogen alpha chain microplate
• Rat Fibrinogen alpha chain standard
• Rat Fibrinogen alpha chain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Fibrinogen alpha chain (FGA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat FGA. No significant cross-reactivity or interference between Rat FGA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Fibrinogen alpha chain is the alpha component of fibrinogen, a blood-borne glycoprotein composed of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus.
Alternative FGA; Fib2; MGC119422; MGC119423; MGC119425; fibrinogen; A alpha polypeptide; fibrinogen; alpha chain; isoform alpha preproprotein; fibrinogen; alpha polypeptide
Accession P14461

Image & description

Fig.1. Rat Fibrinogen alpha chain (FGA) Standard Curve.

Fig.1. Rat Fibrinogen alpha chain (FGA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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