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Rat Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit

Rat Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit

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Specification

Product name Rat Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit employs a two-site sandwich ELISA to quantitate CXCL5 in samples. An antibody specific for CXCL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCXCL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CXCL5; ENA-78; SCYB5; epithelial-derived neutrophil activating protein 78; epithelial-derived neutrophil-activating peptide 78; neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; small ind

Product Properties

Kit components • Rat Epithelial neutrophil activating peptide 78 microplate
• Rat Epithelial neutrophil activating peptide 78 standard
• Rat Epithelial neutrophil activating peptide 78 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Epithelial neutrophil activating peptide 78 (ENA-78) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CXCL5. No significant cross-reactivity or interference between Rat CXCL5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ENA-78 is a CXC chemokine that was originally isolated from media conditioned by the growth of a mouse lung type-II alveolar epithelial cell line (A549) stimulated by IL-1 or TNF. The full-length cDNA encodes a 114 amino acid (aa) residue precursor protein with a 36 aa residue signal peptide that is cleaved to generate the 78 aa residue secreted protein. ENA-78 shares significant amino acid sequence identity with NAP-2 (53%), GRO,, and (52%, 48% and 51%, respectively), and IL-8 (22%). The gene for ENA-78 has been mapped to chromosome 4q13-q21. Like other CXC chemokines, ENA-78 is a neutrophil attractant and activator in vitro. Based on cross-desensitization experiments, it has been suggested that ENA-78 activity can be mediated through the IL-8 receptor system.
Gene ID 60665
Alternative CXCL5; ENA-78; SCYB5; epithelial-derived neutrophil activating protein 78; epithelial-derived neutrophil-activating peptide 78; neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; small ind
Accession P97885

Image & description

Fig.1. Rat Epithelial neutrophil activating peptide 78 (ENA-78) Standard Curve.

Fig.1. Rat Epithelial neutrophil activating peptide 78 (ENA-78) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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