|Product name||Rat Endometrium Antibody (EMAb) ELISA Kit|
|Applications notes||This Rat Endometrium Antibody (EMAb) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EMAb. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated EMAb and incubated. The competitive inhibition reaction is launched between with HRP labeled EMAb and unlabeled EMAb with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of EMAb in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Endometrium Antibody microplate
• Rat Endometrium Antibody standard
• Rat Endometrium Antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Endometrium Antibody (EMAb) ELISA Kit has high sensitivity and excellent specificity for detection of Rat EMAb. No significant cross-reactivity or interference between Rat EMAb and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Anti-endometrial antibodies have been detected in the serum of women with endometriosis using immunofluorescence (Mathur et al, 1982; Wild and Shivers, 1985), immunodiffusion (Badawy et al, 1984), passive haemagglutination (Chihal et al, 1986; Badawy et al, 1990), enzyme-linked immunosorbent assay (ELISA) (Kennedy et al, 1990b) and Western blots (Mathur et al, 1988, 1990). Most studies show a higher prevalence of autoantibodies in women with endometriosis than in controls.However, Switchenko et al (1991), failed to demonstrate consistently higher concentrations of antiendometrial antibodies in women with endometriosis using immunofluorescence, haemagglutination, ELISA, or Western blotting, and concluded mat the case for specific anti-endometrial antibodies remains unproven.|
Fig.1. Rat Endometrium Antibody (EMAb) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.