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Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit

Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit

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Specification

Product name Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DKC1 in samples. An antibody specific for DKC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDKC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DKC1; CBF5; DKC; FLJ97620; NAP57; NOLA4; XAP101; H/ACA ribonucleoprotein complex subunit 4; cbf5p homolog; dyskerin; nopp140-associated protein of 57 kDa; nucleolar protein family A member 4; snoRNP protein DK

Product Properties

Kit components • Rat Dyskeratosis Congenita 1 microplate
• Rat Dyskeratosis Congenita 1 standard
• Rat Dyskeratosis Congenita 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DKC1. No significant cross-reactivity or interference between Rat DKC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dyskeratosis congenita 1, dyskerin,is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins). The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein (MPP1) gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene.
Gene ID 170944
Alternative DKC1; CBF5; DKC; FLJ97620; NAP57; NOLA4; XAP101; H/ACA ribonucleoprotein complex subunit 4; cbf5p homolog; dyskerin; nopp140-associated protein of 57 kDa; nucleolar protein family A member 4; snoRNP protein DK
Accession P40615

Image & description

Fig.1. Rat Dyskeratosis Congenita 1 (DKC1) Standard Curve.

Fig.1. Rat Dyskeratosis Congenita 1 (DKC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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