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Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

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Specification

Product name Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit employs a two-site sandwich ELISA to quantitate POLD1 in samples. An antibody specific for POLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POLD1; CDC2; POLD; CDC2 homolog; DNA-directed DNA polymerase delta 1; DNA-directed polymerase delta 1

Product Properties

Kit components • Rat DNA polymerase delta catalytic subunit microplate
• Rat DNA polymerase delta catalytic subunit standard
• Rat DNA polymerase delta catalytic subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat POLD1. No significant cross-reactivity or interference between Rat POLD1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4. The enzyme is 94% identical to bovine DNA polymerase delta and contains the numerous highly conserved regions previously observed in the bovine and yeast enzymes. The human enzyme also contains 2 putative zinc finger domains in the C-terminal region of the molecule as well as a putative nuclear localization signal at the N-terminal end.WRN functionally interacts with DNA polymerase delta, which is required for DNA replication and DNA repair.
Gene ID 59294
Alternative POLD1; CDC2; POLD; CDC2 homolog; DNA-directed DNA polymerase delta 1; DNA-directed polymerase delta 1
Accession O54747

Image & description

Fig.1. Rat DNA polymerase delta catalytic subunit (POLD1) Standard Curve.

Fig.1. Rat DNA polymerase delta catalytic subunit (POLD1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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