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Rat DNA damage-inducible transcript 4-like protein (DDIT4L) ELISA Kit

Rat DNA damage-inducible transcript 4-like protein (DDIT4L) ELISA Kit

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Specification

Product name Rat DNA damage-inducible transcript 4-like protein (DDIT4L) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat DNA damage-inducible transcript 4-like protein (DDIT4L) ELISA Kit employs a two-site sandwich ELISA to quantitate DDIT4L in samples. An antibody specific for DDIT4L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDIT4L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDIT4L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDIT4L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DDIT4L; REDD2; Rtp801L; homolog of mouse SMHS1; regulated in development and DNA damage response 2

Product Properties

Kit components • Rat DNA damage-inducible transcript 4-like protein microplate
• Rat DNA damage-inducible transcript 4-like protein standard
• Rat DNA damage-inducible transcript 4-like protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat DNA damage-inducible transcript 4-like protein (DDIT4L) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DDIT4L. No significant cross-reactivity or interference between Rat DDIT4L and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.By similarity
Gene ID 100363484
Alternative DDIT4L; REDD2; Rtp801L; homolog of mouse SMHS1; regulated in development and DNA damage response 2
Accession Q8VD50

Image & description

Fig.1. Rat DNA damage-inducible transcript 4-like protein (DDIT4L) Standard Curve.

Fig.1. Rat DNA damage-inducible transcript 4-like protein (DDIT4L) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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