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Rat Disrupted in renal carcinoma protein 2 (DIRC2) ELISA Kit

Rat Disrupted in renal carcinoma protein 2 (DIRC2) ELISA Kit

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Specification

Product name Rat Disrupted in renal carcinoma protein 2 (DIRC2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Disrupted in renal carcinoma protein 2 (DIRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate DIRC2 in samples. An antibody specific for DIRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DIRC2; FLJ14784; RCC4; disrupted in renal cancer protein 2; renal cell carcinoma 4

Product Properties

Kit components • Rat Disrupted in renal carcinoma protein 2 microplate
• Rat Disrupted in renal carcinoma protein 2 standard
• Rat Disrupted in renal carcinoma protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Disrupted in renal carcinoma protein 2 (DIRC2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DIRC2. No significant cross-reactivity or interference between Rat DIRC2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DIRC2 has a conserved motif that is shared with the major facilitator superfamily of transporters between membrane-spanning domains 2 and 3, and a proline-rich region between membrane-spanning domains 6 and 7. DIRC2 also contains a putative N-glycosylation site and several putative phosphorylation sites. Computer predictions of the putative DIRC2 protein showed significant homology to different members of the major facilitator superfamily of transporters. DIRC2 shares 43% similarity with the human homolog of feline leukemia virus type C receptor (FLVXR), which has been classified as a major facilitator superfamily transporter, and more than 85% homology with Dirc2 from monkey, pig, dog, and mouse. Northern blot analysis revealed a 2.2-kb transcript in most tissues tested, including kidney.
Gene ID 303902
Alternative DIRC2; FLJ14784; RCC4; disrupted in renal cancer protein 2; renal cell carcinoma 4
Accession Q66H95

Image & description

Fig.1. Rat Disrupted in renal carcinoma protein 2 (DIRC2) Standard Curve.

Fig.1. Rat Disrupted in renal carcinoma protein 2 (DIRC2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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