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Rat Disks large homolog 4 (DLG4) ELISA Kit

Rat Disks large homolog 4 (DLG4) ELISA Kit

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Specification

Product name Rat Disks large homolog 4 (DLG4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Disks large homolog 4 (DLG4) ELISA Kit employs a two-site sandwich ELISA to quantitate DLG4 in samples. An antibody specific for DLG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLG4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLG4; FLJ97752; FLJ98574; PSD95; SAP-90; SAP90; Tax interaction protein 15; discs large homolog 4; post-synaptic density protein 95; synapse-associated protein 90

Product Properties

Kit components • Rat Disks large homolog 4 microplate
• Rat Disks large homolog 4 standard
• Rat Disks large homolog 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Disks large homolog 4 (DLG4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLG4. No significant cross-reactivity or interference between Rat DLG4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Disks large homolog 4 is a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with DLG2. With DLG2 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. DLG4 is the best studied member of the MAGUK-family of PDZ domain-containing proteins. Like all MAGUK-family proteins, its basic structure includes three PDZ domains, an SH3 domain, and a guanylate kinase-like domain (GK) connected by disordered linker regions. It is almost exclusively located in the post synaptic density of neurons, and is involved in anchoring synaptic proteins.
Gene ID 29495
Alternative DLG4; FLJ97752; FLJ98574; PSD95; SAP-90; SAP90; Tax interaction protein 15; discs large homolog 4; post-synaptic density protein 95; synapse-associated protein 90
Accession P31016

Image & description

Fig.1. Rat Disks large homolog 4 (DLG4) Standard Curve.

Fig.1. Rat Disks large homolog 4 (DLG4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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