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Rat Disks large-associated protein 4 (DLGAP4) ELISA Kit

Rat Disks large-associated protein 4 (DLGAP4) ELISA Kit

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Specification

Product name Rat Disks large-associated protein 4 (DLGAP4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Disks large-associated protein 4 (DLGAP4) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP4 in samples. An antibody specific for DLGAP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLGAP4; DAP4; DLP4; FLJ20588; KIAA0964; MGC131862; RP5-977B1.6; SAPAP4; PSD-95/SAP90-binding protein 4; SAP90/PSD-95-associated protein 4; disks large associated protein 4; disks large-associated protein 4

Product Properties

Kit components • Rat Disks large-associated protein 4 microplate
• Rat Disks large-associated protein 4 standard
• Rat Disks large-associated protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Disks large-associated protein 4 (DLGAP4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLGAP4. No significant cross-reactivity or interference between Rat DLGAP4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DLGAP4 is a membrane-associated guanylate kinase found at the postsynaptic density in neuronal cells. It is a signaling molecule that can interact with potassium channels and receptors, as well as other signaling molecules. The protein encoded by this gene can interact with PSD-95 through its guanylate kinase domain and may be involved in clustering PSD-95 in the postsynaptic density region. The encoded protein is one of at least four similar proteins that have been found. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. SAPAPs are specifically expressed in neuronal cells and enriched in the PSD fraction. SAPAPs induce the enrichment of PSD-95/SAP90 to the plasma membrane in transfected cells.
Gene ID 286930
Alternative DLGAP4; DAP4; DLP4; FLJ20588; KIAA0964; MGC131862; RP5-977B1.6; SAPAP4; PSD-95/SAP90-binding protein 4; SAP90/PSD-95-associated protein 4; disks large associated protein 4; disks large-associated protein 4
Accession P97839

Image & description

Fig.1. Rat Disks large-associated protein 4 (DLGAP4) Standard Curve.

Fig.1. Rat Disks large-associated protein 4 (DLGAP4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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