| Product name | Rat Disks large-associated protein 3 (DLGAP3) ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | This Rat Disks large-associated protein 3 (DLGAP3) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP3 in samples. An antibody specific for DLGAP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | DLGAP3; DAP3; SAPAP3; OTTHUMP00000004180; PSD-95/SAP90-binding protein 3; SAP90/PSD 95 associated protein 3; SAP90/PSD-95-associated protein 3; disks large-associated protein 3 |
| Kit components | • Rat Disks large-associated protein 3 microplate • Rat Disks large-associated protein 3 standard • Rat Disks large-associated protein 3 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Rat Disks large-associated protein 3 (DLGAP3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLGAP3. No significant cross-reactivity or interference between Rat DLGAP3 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Using a segment of rat Fak (PTK2) to screen an adult human brain cDNA library, Bongiorno-Borbone et al. (2005) cloned DLGAP3, which they called SAPAP3. The deduced protein contains 977 amino acids. By Northern blot analysis, Takeuchi et al. (1997) found brain-specific expression of rat Sapap3. The rat protein localized to dendrites and cell bodies and was enriched in synaptosomal membrane fractions.By mutation analysis, Bongiorno-Borbone et al. (2005) demonstrated that the N-terminal half of human SAPAP3 interacted with a C-terminal segment of rat Fak. They also found that SAPAP3 interacted with Pyk2 (PTK2B) in in vitro pull-down assays. In rat forebrain lysates, part of Fak and Pyk2 had a similar subcellular distribution as Sapap3 and Sap90/Psd95, which supported their functional association. |
| Alternative | DLGAP3; DAP3; SAPAP3; OTTHUMP00000004180; PSD-95/SAP90-binding protein 3; SAP90/PSD 95 associated protein 3; SAP90/PSD-95-associated protein 3; disks large-associated protein 3 |
| Accession | P97838 |
Fig.1. Rat Disks large-associated protein 3 (DLGAP3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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