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Rat Disks large-associated protein 1 (DLGAP1) ELISA Kit

Rat Disks large-associated protein 1 (DLGAP1) ELISA Kit

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Specification

Product name Rat Disks large-associated protein 1 (DLGAP1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Disks large-associated protein 1 (DLGAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP1 in samples. An antibody specific for DLGAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLGAP1; DAP-1; DAP-1-ALPHA; DAP-1-BETA; GKAP; MGC88156; SAPAP1; hGKAP; PSD-95/SAP90 binding protein 1; SAP90/PSD-95-associated protein 1; discs large homolog-associated protein 1; disks large-associated protei

Product Properties

Kit components • Rat Disks large-associated protein 1 microplate
• Rat Disks large-associated protein 1 standard
• Rat Disks large-associated protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Disks large-associated protein 1 (DLGAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLGAP1. No significant cross-reactivity or interference between Rat DLGAP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DLGAP1encodes the protein called guanylate kinase-associated protein (GKAP). GKAP binds to the SHANK and PSD-95 proteins fascilitating the assembly of the post synaptic density of neurons. Sequence analysis predicted that the 627-amino acid hydrophilic protein, which is 98% identical to the rat sequence, contains five 14-amino acid repeats in its N terminus. Binding analysis determined that the N-terminal region of DLGAP1 interacts with intact GK domains of all members of the DLG family, which share approximately 75% homology in the GK domain; DLGAP1 was found to interact less well with more distantly related MAGUKs. Immunoblot analysis showed expression of 95- and 130-kD DLGAP1 proteins in membranes from different regions of rat brain.
Gene ID 65040
Alternative DLGAP1; DAP-1; DAP-1-ALPHA; DAP-1-BETA; GKAP; MGC88156; SAPAP1; hGKAP; PSD-95/SAP90 binding protein 1; SAP90/PSD-95-associated protein 1; discs large homolog-associated protein 1; disks large-associated protei
Accession P97836

Image & description

Fig.1. Rat Disks large-associated protein 1 (DLGAP1) Standard Curve.

Fig.1. Rat Disks large-associated protein 1 (DLGAP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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