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Rat DIS3-like exonuclease 1 (DIS3L) ELISA Kit

Rat DIS3-like exonuclease 1 (DIS3L) ELISA Kit

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Specification

Product name Rat DIS3-like exonuclease 1 (DIS3L) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat DIS3-like exonuclease 1 (DIS3L) ELISA Kit employs a two-site sandwich ELISA to quantitate DIS3L in samples. An antibody specific for DIS3L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIS3L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIS3L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIS3L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DIS3L; FLJ38088; KIAA1955; MGC4562;

Product Properties

Kit components • Rat DIS3-like exonuclease 1 microplate
• Rat DIS3-like exonuclease 1 standard
• Rat DIS3-like exonuclease 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat DIS3-like exonuclease 1 (DIS3L) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DIS3L. No significant cross-reactivity or interference between Rat DIS3L and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dis3L is an RNase enzyme homologous to the yeast protein Rrp44, and can be part of the exosome complex in the cytoplasm of eukaryotic cells. DIS3L belongs to the ribonuclease II (RNB) family. Ribonuclease II (RNase II) is a major exoribonuclease that intervenes in all of these fundamental processes; it can act independently or as a component of the exosome, an essential RNA-degrading multiprotein complex. RNase II-like enzymes are found in all three kingdoms of life, but there are no structural data for any of the proteins of this family. RNase R is a close homolog of RNase II, but it can, unlike RNase II, degrade RNA with secondary structures without help of accessory factors.
Gene ID 363077
Alternative DIS3L; FLJ38088; KIAA1955; MGC4562;
Accession Q5U2P0

Image & description

Fig.1. Rat DIS3-like exonuclease 1 (DIS3L) Standard Curve.

Fig.1. Rat DIS3-like exonuclease 1 (DIS3L) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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