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Rat Dipeptidyl peptidase 4 (DPP4) ELISA Kit

Rat Dipeptidyl peptidase 4 (DPP4) ELISA Kit

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Specification

Product name Rat Dipeptidyl peptidase 4 (DPP4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Dipeptidyl peptidase 4 (DPP4) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP4 in samples. An antibody specific for DPP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DPP4; ADABP; ADCP2; CD26; DPPIV; TP103; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase 4; dipeptidylpeptidase IV; dipeptidylpeptidase IV (CD26; adenosine deamina

Product Properties

Kit components • Rat Dipeptidyl peptidase 4 microplate
• Rat Dipeptidyl peptidase 4 standard
• Rat Dipeptidyl peptidase 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Dipeptidyl peptidase 4 (DPP4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DPP4. No significant cross-reactivity or interference between Rat DPP4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by the DPPIV gene is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPPⅣ plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1.Furthermore it appears to work as a suppressor in the development of cancer and tumours.CD26/DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum being increased in some neoplasms and decreased in others.
Gene ID 25253
Alternative DPP4; ADABP; ADCP2; CD26; DPPIV; TP103; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase 4; dipeptidylpeptidase IV; dipeptidylpeptidase IV (CD26; adenosine deamina
Accession P14740

Image & description

Fig.1. Rat Dipeptidyl peptidase 4 (DPP4) Standard Curve.

Fig.1. Rat Dipeptidyl peptidase 4 (DPP4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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