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Rat Dihydropyrimidine dehydrogenase (DPYD) ELISA Kit

Rat Dihydropyrimidine dehydrogenase (DPYD) ELISA Kit

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Specification

Product name Rat Dihydropyrimidine dehydrogenase (DPYD) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Dihydropyrimidine dehydrogenase (DPYD) ELISA Kit employs a two-site sandwich ELISA to quantitate DPYD in samples. An antibody specific for DPYD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPYD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPYD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPYD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DPYD; DHP; DHPDHASE; DPD; MGC132008; MGC70799; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase

Product Properties

Kit components • Rat Dihydropyrimidine dehydrogenase microplate
• Rat Dihydropyrimidine dehydrogenase standard
• Rat Dihydropyrimidine dehydrogenase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Dihydropyrimidine dehydrogenase (DPYD) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DPYD. No significant cross-reactivity or interference between Rat DPYD and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dihydropyrimidine dehydrogenase (DPD) is an enzyme that is involved in pyrimidine degradation. It is the initial and rate-limiting step in pyrimidine catabolism. It catalyzes the reduction of uracil and thymine. It is also involved in the degradation of the chemotherapeutic drugs 5-fluorouracil and Tegafur-uracil.The sequence of the gene suggested that DPD has at least 3 distinct domains: a possible NADPH binding site and FAD-binding site in the N terminus, 2 motifs of putative iron/sulfur-binding sites near the C terminus, and a peptide domain corresponding to the uracil-binding site. Expression of the pig enzyme in E. coli catalyzed the reduction of uracil, thymine, and 5-fluorouracil (5FU) with kinetics approximating those published for the enzyme purified from mammalian liver.
Gene ID 81656
Alternative DPYD; DHP; DHPDHASE; DPD; MGC132008; MGC70799; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase
Accession O89000

Image & description

Fig.1. Rat Dihydropyrimidine dehydrogenase (DPYD) Standard Curve.

Fig.1. Rat Dihydropyrimidine dehydrogenase (DPYD) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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