Login Register
English
0

Cart

$ 0

Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit

Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit

Views(92) Publications(0) Catalog no(KTE100760)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit employs a two-site sandwich ELISA to quantitate DLAT in samples. An antibody specific for DLAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLAT; DLTA; PDC-E2; PDCE2; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit

Product Properties

Kit components • Rat Dihydrolipoyl Transacetylase microplate
• Rat Dihydrolipoyl Transacetylase standard
• Rat Dihydrolipoyl Transacetylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLAT. No significant cross-reactivity or interference between Rat DLAT and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dihydrolipoyl transacetylase is the component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.
Gene ID 81654
Alternative DLAT; DLTA; PDC-E2; PDCE2; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit
Accession P08461

Image & description

Fig.1. Rat Dihydrolipoyl Transacetylase (DLAT) Standard Curve.

Fig.1. Rat Dihydrolipoyl Transacetylase (DLAT) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit”