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Rat Diamine oxidase (DAO) ELISA Kit

Rat Diamine oxidase (DAO) ELISA Kit

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Specification

Product name Rat Diamine oxidase (DAO) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Diamine oxidase (DAO) ELISA Kit employs a two-site sandwich ELISA to quantitate DAO in samples. An antibody specific for DAO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDAO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DAO; DAAO; DAMOX; MGC35381; OXDA;

Product Properties

Kit components • Rat Diamine oxidase microplate
• Rat Diamine oxidase standard
• Rat Diamine oxidase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Diamine oxidase (DAO) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DAO. No significant cross-reactivity or interference between Rat DAO and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background On the basis of its primary structure, the amiloride-binding protein (EC 1.4.3.6) is 713 amino acids long, with a 19-amino acid signal peptide. Expressed in cultured cells, the mRNA yields a glycoprotein that binds amiloride and amiloride analogs with affinities similar to the amiloride receptor associated with the apical Na+ channel in pig kidney membranes and is immunoprecipitated with monoclonal antibodies raised against pig kidney amiloride-binding protein. Barbry et al. (1990) pointed out that amiloride-sensitive Na+ channels are also present in airway epithelia, where they play an important role in fluid secretion. Amiloride inhibits the excessive absorption of Na+ and liquid that takes place in airway epithelia of patients with cystic fibrosis, and amiloride aerosol therapy has been tried for the treatment of lung disease in CF.
Gene ID 100008977
Alternative DAO; DAAO; DAMOX; MGC35381; OXDA;
Accession P22942

Image & description

Fig.1. Rat Diamine oxidase (DAO) Standard Curve.

Fig.1. Rat Diamine oxidase (DAO) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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