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Rat Diacylglycerol kinase beta (DGKB) ELISA Kit

Rat Diacylglycerol kinase beta (DGKB) ELISA Kit

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Specification

Product name Rat Diacylglycerol kinase beta (DGKB) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Diacylglycerol kinase beta (DGKB) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKB in samples. An antibody specific for DGKB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGKB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DGKB; DAGK2; DGK; DGK-BETA; KIAA0718; DAG kinase beta; OTTHUMP00000201739; diacylglycerol kinase; beta; diglyceride kinase beta

Product Properties

Kit components • Rat Diacylglycerol kinase beta microplate
• Rat Diacylglycerol kinase beta standard
• Rat Diacylglycerol kinase beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Diacylglycerol kinase beta (DGKB) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DGKB. No significant cross-reactivity or interference between Rat DGKB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by DGKB is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for DGKB.
Gene ID 54248
Alternative DGKB; DAGK2; DGK; DGK-BETA; KIAA0718; DAG kinase beta; OTTHUMP00000201739; diacylglycerol kinase; beta; diglyceride kinase beta
Accession P49621

Image & description

Fig.1. Rat Diacylglycerol kinase beta (DGKB) Standard Curve.

Fig.1. Rat Diacylglycerol kinase beta (DGKB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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