Login Register


$ 0

Rat Deoxycytidine kinase (DCK) ELISA Kit

Rat Deoxycytidine kinase (DCK) ELISA Kit

Views(169) Publications(0) Catalog no(KTE100791)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)


Product name Rat Deoxycytidine kinase (DCK) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Deoxycytidine kinase (DCK) ELISA Kit employs a two-site sandwich ELISA to quantitate DCK in samples. An antibody specific for DCK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DCK; MGC117410; MGC138632;

Product Properties

Kit components • Rat Deoxycytidine kinase microplate
• Rat Deoxycytidine kinase standard
• Rat Deoxycytidine kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Deoxycytidine kinase (DCK) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DCK. No significant cross-reactivity or interference between Rat DCK and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.
Gene ID 79127
Alternative DCK; MGC117410; MGC138632;
Accession P48769

Image & description

Fig.1. Rat Deoxycytidine kinase (DCK) Standard Curve.

Fig.1. Rat Deoxycytidine kinase (DCK) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications


There are no reviews yet.

Be the first to review “Rat Deoxycytidine kinase (DCK) ELISA Kit”