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Rat Dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit

Rat Dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit

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Specification

Product name Rat Dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMP1 in samples. An antibody specific for DMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DMP1; ARHP; ARHR; DMP-1; dentin matrix protein 1

Product Properties

Kit components • Rat Dentin matrix acidic phosphoprotein 1 microplate
• Rat Dentin matrix acidic phosphoprotein 1 standard
• Rat Dentin matrix acidic phosphoprotein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DMP1. No significant cross-reactivity or interference between Rat DMP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DMP1 is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.
Alternative DMP1; ARHP; ARHR; DMP-1; dentin matrix protein 1
Accession P98193

Image & description

Fig.1. Rat Dentin matrix acidic phosphoprotein 1 (DMP1) Standard Curve.

Fig.1. Rat Dentin matrix acidic phosphoprotein 1 (DMP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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