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Rat Delta-like protein 3 (DLL3) ELISA Kit

Rat Delta-like protein 3 (DLL3) ELISA Kit

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Specification

Product name Rat Delta-like protein 3 (DLL3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Delta-like protein 3 (DLL3) ELISA Kit employs a two-site sandwich ELISA to quantitate DLL3 in samples. An antibody specific for DLL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLL3; SCDO1; delta-like 3 protein

Product Properties

Kit components • Rat Delta-like protein 3 microplate
• Rat Delta-like protein 3 standard
• Rat Delta-like protein 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Delta-like protein 3 (DLL3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLL3. No significant cross-reactivity or interference between Rat DLL3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. Disruption of the Notch pathway occurs in Alagille syndrome , a disorder that has vertebral abnormalities, i.e., 'butterfly vertebrae,' as a feature in about two-thirds of patients.
Gene ID 114125
Alternative DLL3; SCDO1; delta-like 3 protein
Accession O88671

Image & description

Fig.1. Rat Delta-like protein 3 (DLL3) Standard Curve.

Fig.1. Rat Delta-like protein 3 (DLL3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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