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Rat Delta (24)-sterol reductase (DHCR24) ELISA Kit

Rat Delta (24)-sterol reductase (DHCR24) ELISA Kit

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Specification

Product name Rat Delta (24)-sterol reductase (DHCR24) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Delta (24)-sterol reductase (DHCR24) ELISA Kit employs a two-site sandwich ELISA to quantitate DHCR24 in samples. An antibody specific for DHCR24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHCR24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DHCR24; KIAA0018; Nbla03646; SELADIN1; seladin-1; 3 beta-hydroxysterol delta 24-reductase; diminuto/dwarf1 homolog; putative protein product of Nbla03646; selective AD indicator 1

Product Properties

Kit components • Rat Delta microplate
• Rat Delta standard
• Rat Delta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Delta (24)-sterol reductase (DHCR24) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DHCR24. No significant cross-reactivity or interference between Rat DHCR24 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 have been associated with desmosterolosis. Also, reduced expression of DHCR24 occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
Alternative DHCR24; KIAA0018; Nbla03646; SELADIN1; seladin-1; 3 beta-hydroxysterol delta 24-reductase; diminuto/dwarf1 homolog; putative protein product of Nbla03646; selective AD indicator 1
Accession Q5BQE6

Image & description

Fig.1. Rat Delta (24)-sterol reductase (DHCR24) Standard Curve.

Fig.1. Rat Delta (24)-sterol reductase (DHCR24) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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