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Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit

Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit

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Specification

Product name Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLC1 in samples. An antibody specific for DLC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLC1; ARHGAP7; FLJ21120; HP; STARD12; p122-RhoGAP; Rho-GTPase-activating protein 7; StAR-related lipid transfer (START) domain containing 12; deleted in liver cancer 1 variant 2

Product Properties

Kit components • Rat Deleted In Liver Cancer 1 microplate
• Rat Deleted In Liver Cancer 1 standard
• Rat Deleted In Liver Cancer 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLC1. No significant cross-reactivity or interference between Rat DLC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Deleted in Liver Cancer 1 is deleted in the primary tumor of hepatocellular carcinoma. It maps to 8p22-p21.3, a region frequently deleted in solid tumors. It is suggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.DLC1 is frequently inactivated in human hepatocellular carcinoma, as well as some nasopharyngeal, lung, breast, prostate, kidney, colon, uterine, ovarian, and gastric cancers. The DLC1 protein contains four major functional domains: an N-terminal sterile α motif (SAM), a serine-rich (SR) region, a Rho-GAP domain, and a C-terminal steroidogenic acute regulatory protein related lipid-transfer (START) domain. DLC1 is localized to focal adhesions located at the periphery of cells.
Alternative DLC1; ARHGAP7; FLJ21120; HP; STARD12; p122-RhoGAP; Rho-GTPase-activating protein 7; StAR-related lipid transfer (START) domain containing 12; deleted in liver cancer 1 variant 2
Accession Q63744

Image & description

Fig.1. Rat Deleted In Liver Cancer 1 (DLC1) Standard Curve.

Fig.1. Rat Deleted In Liver Cancer 1 (DLC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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