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Rat Death domain-associated protein 6 (DAXX) ELISA Kit

Rat Death domain-associated protein 6 (DAXX) ELISA Kit

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Specification

Product name Rat Death domain-associated protein 6 (DAXX) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Death domain-associated protein 6 (DAXX) ELISA Kit employs a two-site sandwich ELISA to quantitate DAXX in samples. An antibody specific for DAXX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDAXX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAXX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAXX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DAXX; DADB-159G18.9; BING2; DAP6; EAP1; MGC126245; MGC126246; CENP-C binding protein; ETS1-associated protein 1; Fas-binding protein; death-associated protein 6

Product Properties

Kit components • Rat Death domain-associated protein 6 microplate
• Rat Death domain-associated protein 6 standard
• Rat Death domain-associated protein 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Death domain-associated protein 6 (DAXX) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DAXX. No significant cross-reactivity or interference between Rat DAXX and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Death domain-associated protein 6 (DAXX) encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
Alternative DAXX; DADB-159G18.9; BING2; DAP6; EAP1; MGC126245; MGC126246; CENP-C binding protein; ETS1-associated protein 1; Fas-binding protein; death-associated protein 6
Accession Q8VIB2

Image & description

Fig.1. Rat Death domain-associated protein 6 (DAXX) Standard Curve.

Fig.1. Rat Death domain-associated protein 6 (DAXX) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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