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Rat DAZ-associated protein 2 (DAZAP2) ELISA Kit

Rat DAZ-associated protein 2 (DAZAP2) ELISA Kit

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Specification

Product name Rat DAZ-associated protein 2 (DAZAP2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat DAZ-associated protein 2 (DAZAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate DAZAP2 in samples. An antibody specific for DAZAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDAZAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAZAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAZAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DAZAP2; KIAA0058; MGC14319; MGC766; PRTB; deleted in azoospermia associated protein 2; proline-rich transcript in brain; proline-rich transcript; brain-expressed protein

Product Properties

Kit components • Rat DAZ-associated protein 2 microplate
• Rat DAZ-associated protein 2 standard
• Rat DAZ-associated protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat DAZ-associated protein 2 (DAZAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DAZAP2. No significant cross-reactivity or interference between Rat DAZAP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DAZAP2 encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability in splicing factor containing nuclear speckles. The encoded protein may function in various biological and pathological processes including spermatogenesis, cell signaling and transcription regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis of multiple myeloma. Multiple transcript variants encoding different isoforms have been found for DAZAP2.
Gene ID 300235
Alternative DAZAP2; KIAA0058; MGC14319; MGC766; PRTB; deleted in azoospermia associated protein 2; proline-rich transcript in brain; proline-rich transcript; brain-expressed protein
Accession P60486

Image & description

Fig.1. Rat DAZ-associated protein 2 (DAZAP2) Standard Curve.

Fig.1. Rat DAZ-associated protein 2 (DAZAP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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