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Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit

Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit

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Specification

Product name Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP7A1 in samples. An antibody specific for CYP7A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP7A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CYP7A1; CP7A; CYP7; MGC126826; MGC138389; cholesterol 7 alpha-monooxygenase; cholesterol 7-alpha-hydroxylase; cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1

Product Properties

Kit components • Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 microplate
• Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 standard
• Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CYP7A1. No significant cross-reactivity or interference between Rat CYP7A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CYP7A1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of CYP7A1 are associated with defects in bile acid synthesis.
Gene ID 100328551
Alternative CYP7A1; CP7A; CYP7; MGC126826; MGC138389; cholesterol 7 alpha-monooxygenase; cholesterol 7-alpha-hydroxylase; cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1
Accession P51542

Image & description

Fig.1. Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) Standard Curve.

Fig.1. Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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