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Rat Cytochrome c (CYCS) ELISA Kit

Rat Cytochrome c (CYCS) ELISA Kit

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Product name Rat Cytochrome c (CYCS) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Cytochrome c (CYCS) ELISA Kit employs a two-site sandwich ELISA to quantitate CYCS in samples. An antibody specific for CYCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYCS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYCS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CYCS; CYC; HCS; THC4; OTTHUMP00000202043; OTTHUMP00000202044; cytochrome c

Product Properties

Kit components • Rat Cytochrome c microplate
• Rat Cytochrome c standard
• Rat Cytochrome c detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Cytochrome c (CYCS) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CYCS. No significant cross-reactivity or interference between Rat CYCS and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cytochrome c, or cyt c (horse heart: PDB 1HRC) is a small heme protein found loosely associated with the inner membrane of the mitochondrion. It is a soluble protein, unlike other cytochromes, and is an essential component of the electron transfer chain. It is capable of undergoing oxidation and reduction, but does not bind oxygen. It transfers electrons between Complexes III and IV. Cytochrome c is a highly conserved protein across the spectrum of species, found in plants, animals, and many unicellular organisms. This, along with its small size (molecular weight about 12,000 daltons), makes it useful in studies of evolutionary divergence. Its primary structure consists of a chain of 100 amino acids.
Alternative CYCS; CYC; HCS; THC4; OTTHUMP00000202043; OTTHUMP00000202044; cytochrome c
Accession P00008

Image & description

Fig.1. Rat Cytochrome c (CYCS) Standard Curve.

Fig.1. Rat Cytochrome c (CYCS) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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