| Product name | Rat Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | This Rat Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5R4 in samples. An antibody specific for CYB5R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYB5R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5R4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | CYB5R4; NCB5OR; RP4-676J13.1; dJ676J13.1; NADPH cytochrome B5 oxidoreductase; cytochrome b-type NAD(P)H oxidoreductase; flavohemoprotein b5+b5R |
| Kit components | • Rat Cytochrome b5 reductase 4 microplate • Rat Cytochrome b5 reductase 4 standard • Rat Cytochrome b5 reductase 4 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Rat Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CYB5R4. No significant cross-reactivity or interference between Rat CYB5R4 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cytochrome b5 reductase 4 is an enzyme that in humans is encoded by the CYB5R4 gene. NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5; MIM 250790) and CYB5 reductase. |
| Gene ID | 171015 |
| Alternative | CYB5R4; NCB5OR; RP4-676J13.1; dJ676J13.1; NADPH cytochrome B5 oxidoreductase; cytochrome b-type NAD(P)H oxidoreductase; flavohemoprotein b5+b5R |
| Accession | Q68EJ0 |
Fig.1. Rat Cytochrome b5 reductase 4 (CYB5R4) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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