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Rat Cluster of differentiation 45 (CD45) ELISA Kit

Rat Cluster of differentiation 45 (CD45) ELISA Kit

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Specification

Product name Rat Cluster of differentiation 45 (CD45) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Cluster of differentiation 45 (CD45) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRC in samples. An antibody specific for PTPRC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTPRC; B220; CD45; CD45R; GP180; LCA; LY5; T200; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; glycoprotein; leukocyte-common antigen; protein tyrosine phosphatase; receptor type; c polypeptid

Product Properties

Kit components • Rat Cluster of differentiation 45 microplate
• Rat Cluster of differentiation 45 standard
• Rat Cluster of differentiation 45 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Cluster of differentiation 45 (CD45) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTPRC. No significant cross-reactivity or interference between Rat PTPRC and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by CD45 gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
Gene ID 24699
Alternative PTPRC; B220; CD45; CD45R; GP180; LCA; LY5; T200; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; glycoprotein; leukocyte-common antigen; protein tyrosine phosphatase; receptor type; c polypeptid
Accession P04157

Image & description

Fig.1. Rat Cluster of differentiation 45 (CD45) Standard Curve.

Fig.1. Rat Cluster of differentiation 45 (CD45) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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