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Rat CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit

Rat CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit

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Specification

Product name Rat CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKG2 in samples. An antibody specific for PRKG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRKG2; PRKGR2; cGKII; OTTHUMP00000160807

Product Properties

Kit components • Rat CGMP-dependent protein kinase 2 microplate
• Rat CGMP-dependent protein kinase 2 standard
• Rat CGMP-dependent protein kinase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRKG2. No significant cross-reactivity or interference between Rat PRKG2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pfeifer et al. (1996) noted that there are 2 forms of cGMP-dependent protein kinase (cGK), types I and II, which are encoded by distinct genes. Smooth muscle, platelets, and cerebellum contain high concentrations of cGKI, whereas cGKII is highly concentrated in brain, lung, and intestinal mucosa. Orstavik et al. (1996) cloned a human cDNA encoding type II cGK by using the mouse type II cGK cDNA to probe a cerebellum cDNA library. The 762-amino acid human type II cGK protein is 96% identical to the mouse and rat type II cGK proteins. Human type II cGK is expressed as a 6-kb mRNA in prostate, small intestine, and colon and as a 4.4-kb mRNA in thymus and prostate.
Gene ID 25523
Alternative PRKG2; PRKGR2; cGKII; OTTHUMP00000160807
Accession Q64595

Image & description

Fig.1. Rat CGMP-dependent protein kinase 2 (PRKG2) Standard Curve.

Fig.1. Rat CGMP-dependent protein kinase 2 (PRKG2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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