| Product name | Rat Caspase-3 (CASP3) ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | This Rat Caspase-3 (CASP3) ELISA Kit employs a two-site sandwich ELISA to quantitate CASP3 in samples. An antibody specific for CASP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCASP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASP3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | CASP3; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; caspase 3; caspase 3; apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3 |
| Kit components | • Rat Caspase-3 microplate • Rat Caspase-3 standard • Rat Caspase-3 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Rat Caspase-3 (CASP3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CASP3. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Caspase-3 (CPP-32, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. |
| Gene ID | 100008840 |
| Alternative | CASP3; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; caspase 3; caspase 3; apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3 |
| Accession | Q8MJC3 |
Fig.1. Rat Caspase-3 (CASP3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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