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Rat CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit

Rat CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit

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Specification

Product name Rat CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2B in samples. An antibody specific for PRKAR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRKAR2B; PRKAR2; RII-BETA; H_RG363E19.2; WUGSC:H_RG363E19.2; cAMP-dependent protein kinase type-beta regulatory chain; cAMP-dependent protein kinase; regulatory subunit beta 2

Product Properties

Kit components • Rat CAMP-dependent protein kinase type II-beta regulatory subunit microplate
• Rat CAMP-dependent protein kinase type II-beta regulatory subunit standard
• Rat CAMP-dependent protein kinase type II-beta regulatory subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRKAR2B. No significant cross-reactivity or interference between Rat PRKAR2B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKAR2b is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity.
Gene ID 24679
Alternative PRKAR2B; PRKAR2; RII-BETA; H_RG363E19.2; WUGSC:H_RG363E19.2; cAMP-dependent protein kinase type-beta regulatory chain; cAMP-dependent protein kinase; regulatory subunit beta 2
Accession P12369

Image & description

Fig.1. Rat CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) Standard Curve.

Fig.1. Rat CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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