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Rat CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit

Rat CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit

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Specification

Product name Rat CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACA in samples. An antibody specific for PRKACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRKACA; MGC102831; MGC48865; PKACA; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha; isoform 1; protein kinase A catalytic subunit

Product Properties

Kit components • Rat CAMP-dependent protein kinase catalytic subunit alpha microplate
• Rat CAMP-dependent protein kinase catalytic subunit alpha standard
• Rat CAMP-dependent protein kinase catalytic subunit alpha detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRKACA. No significant cross-reactivity or interference between Rat PRKACA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACais a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alternative PRKACA; MGC102831; MGC48865; PKACA; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha; isoform 1; protein kinase A catalytic subunit
Accession P27791

Image & description

Fig.1. Rat CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) Standard Curve.

Fig.1. Rat CAMP-dependent protein kinase catalytic subunit alpha (PRKACA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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