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Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit

Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit

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Specification

Product name Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP3R1 in samples. An antibody specific for PPP3R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP3R1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP3R1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP3R1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PPP3R1; CALNB1; CNB; CNB1; calcineurin B; type I (19kDa); protein phosphatase 3 (formerly 2B); regulatory subunit B (19kD); alpha isoform (calcineurin B; type I); protein phosphatase 3 (formerly 2B); regulato

Product Properties

Kit components • Rat Calcineurin subunit B type 1 microplate
• Rat Calcineurin subunit B type 1 standard
• Rat Calcineurin subunit B type 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PPP3R1. No significant cross-reactivity or interference between Rat PPP3R1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca(2+)-binding regulatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha and beta, of calcineurin A encoded by separate genes located on different human chromosomes. A third isoform, A-gamma, is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs. Calcineurin is especially abundant in brain where it constitutes 1% of total protein.
Gene ID 29748
Alternative PPP3R1; CALNB1; CNB; CNB1; calcineurin B; type I (19kDa); protein phosphatase 3 (formerly 2B); regulatory subunit B (19kD); alpha isoform (calcineurin B; type I); protein phosphatase 3 (formerly 2B); regulato
Accession P63100

Image & description

Fig.1. Rat Calcineurin subunit B type 1 (PPP3R1) Standard Curve.

Fig.1. Rat Calcineurin subunit B type 1 (PPP3R1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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