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Rat C-C motif chemokine 6 (CCL6) ELISA Kit

Rat C-C motif chemokine 6 (CCL6) ELISA Kit

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Specification

Product name Rat C-C motif chemokine 6 (CCL6) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat C-C motif chemokine 6 (CCL6) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL6 in samples. An antibody specific for CCL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CCL6; Small-inducible cytokine A6

Product Properties

Kit components • Rat C-C motif chemokine 6 microplate
• Rat C-C motif chemokine 6 standard
• Rat C-C motif chemokine 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat C-C motif chemokine 6 (CCL6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CCL6. No significant cross-reactivity or interference between Rat CCL6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background C10, known also as SCYA6 (small inducible cytokine A6) is one of several mRNAs that are acutely expressed in myelopoietic mouse bone marrow cultures stimulated by GM-CSF. It is also strongly elevated during the induction of neutrophilic differentiation of 32D cells that are dependent on the presence of IL3 or G-CSF.C10 is related to MIP-1. Its protein sequence demonstrates that C10 belongs to the family of chemotactic cytokines known as Chemokines (beta-Chemokines or CC-Chemokines). The protein contains six instead of four conserved cysteine residues. The C10 gene contains a second exon not found in other Chemokines. This exon encodes a 16 amino acid sequence, which is inserted into the amino terminal region of the protein and plays a key role in recognizing or activating chemokine receptors.
Alternative CCL6; Small-inducible cytokine A6

Image & description

Fig.1. Rat C-C motif chemokine 6 (CCL6) Standard Curve.

Fig.1. Rat C-C motif chemokine 6 (CCL6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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