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Rat Bile acid receptor (NR1H4) ELISA Kit

Rat Bile acid receptor (NR1H4) ELISA Kit

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Specification

Product name Rat Bile acid receptor (NR1H4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Bile acid receptor (NR1H4) ELISA Kit employs a two-site sandwich ELISA to quantitate NR1H4 in samples. An antibody specific for NR1H4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNR1H4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NR1H4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NR1H4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NR1H4; BAR; FXR; HRR-1; HRR1; MGC163445; RIP14; farnesoid X receptor

Product Properties

Kit components • Rat Bile acid receptor microplate
• Rat Bile acid receptor standard
• Rat Bile acid receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Bile acid receptor (NR1H4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NR1H4. No significant cross-reactivity or interference between Rat NR1H4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The farnesoid X receptor (FXR),is a nuclear hormone receptor with activity similar to that seen in other steroid receptors such as estrogen or progesterone but more similar in form to PPAR, LXR and RXR. FXR is encoded by the NR1H4 gene.FXR is expressed at high levels in the liver and intestine. Chenodeoxycholic acid and other bile acids are natural ligands for FXR. Like other steroid receptors, when activated, FXR translocates to the cell nucleus, forms a dimer (in this case a heterodimer with RXR) and binds to hormone response elements on DNA which elicits expression or transrepression of gene products.
Gene ID 60351
Alternative NR1H4; BAR; FXR; HRR-1; HRR1; MGC163445; RIP14; farnesoid X receptor
Accession Q62735

Image & description

Fig.1. Rat Bile acid receptor (NR1H4) Standard Curve.

Fig.1. Rat Bile acid receptor (NR1H4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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