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Rat Bifunctional protein NCOAT (MGEA5) ELISA Kit

Rat Bifunctional protein NCOAT (MGEA5) ELISA Kit

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Specification

Product name Rat Bifunctional protein NCOAT (MGEA5) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Bifunctional protein NCOAT (MGEA5) ELISA Kit employs a two-site sandwich ELISA to quantitate MGEA5 in samples. An antibody specific for MGEA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGEA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGEA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGEA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MGEA5; FLJ11229; FLJ23355; KIAA0679; MEA5; NCOAT; OGA; O-GlcNAcase; hyaluronidase in meningioma; nuclear cytoplasmic O-GlcNAcase and acetyltransferase

Product Properties

Kit components • Rat Bifunctional protein NCOAT microplate
• Rat Bifunctional protein NCOAT standard
• Rat Bifunctional protein NCOAT detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Bifunctional protein NCOAT (MGEA5) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MGEA5. No significant cross-reactivity or interference between Rat MGEA5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications.
Gene ID 154968
Alternative MGEA5; FLJ11229; FLJ23355; KIAA0679; MEA5; NCOAT; OGA; O-GlcNAcase; hyaluronidase in meningioma; nuclear cytoplasmic O-GlcNAcase and acetyltransferase
Accession Q8VIJ5

Image & description

Fig.1. Rat Bifunctional protein NCOAT (MGEA5) Standard Curve.

Fig.1. Rat Bifunctional protein NCOAT (MGEA5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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