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Rat Beta-thromboglobulin (BTG) ELISA Kit

Rat Beta-thromboglobulin (BTG) ELISA Kit

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Specification

Product name Rat Beta-thromboglobulin (BTG) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Beta-thromboglobulin (BTG) ELISA Kit employs a two-site sandwich ELISA to quantitate B-TG in samples. An antibody specific for B-TG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB-TG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B-TG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B-TG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative B-TG; CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Chemokine C-X-C-Motif Ligand 7 Chemokine (C-X-C motif) ligand 7 (CXCL7) is a platelet-derived growth factor that belongs to the CXC chemokine family. It is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein (PPBP).

Product Properties

Kit components • Rat Beta-thromboglobulin microplate
• Rat Beta-thromboglobulin standard
• Rat Beta-thromboglobulin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Beta-thromboglobulin (BTG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat B-TG. No significant cross-reactivity or interference between Rat B-TG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Chemokine (C-X-C motif) ligand 7 (CXCL11) is a platelet-derived growth factor that belongs to the CXC chemokine family. It is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein (PPBP).Beta-thromboglobulin (β-TG) has a molecular weight of 35000 daltons and is a specific protein of platelets. Composed of 4 identical subunits; It is synthesized in the cells as a biologically inactive 15 kDa precursor called PBP (platelet basic protein). It is stored in the Alpha-granules of platelets and released in large amounts after platelet activation.It is a protein that is released in large amounts from platelets following their activation. It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.
Alternative B-TG; CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Chemokine C-X-C-Motif Ligand 7 Chemokine (C-X-C motif) ligand 7 (CXCL7) is a platelet-derived growth factor that belongs to the CXC chemokine family. It is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein (PPBP).

Image & description

Fig.1. Rat Beta-thromboglobulin (BTG) Standard Curve.

Fig.1. Rat Beta-thromboglobulin (BTG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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