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Rat Basigin (BSG) ELISA Kit

Rat Basigin (BSG) ELISA Kit

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Specification

Product name Rat Basigin (BSG) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Basigin (BSG) ELISA Kit employs a two-site sandwich ELISA to quantitate BSG in samples. An antibody specific for BSG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBSG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BSG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BSG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative BSG; 5F7; CD147; EMMPRIN; M6; OK; TCSF; CD147 antigen; M6 leukocyte activation antigen; OK blood group antigen; basigin; collagenase stimulatory factor; extracellular matrix metalloproteinase inducer

Product Properties

Kit components • Rat Basigin microplate
• Rat Basigin standard
• Rat Basigin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Basigin (BSG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat BSG. No significant cross-reactivity or interference between Rat BSG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Basigin is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition.It has a variety of functions. In addition to its metalloproteinase-inducing ability, basigin also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes.Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin proteins Cyp-A and CyP-B and certain integrins.
Gene ID 100009229
Alternative BSG; 5F7; CD147; EMMPRIN; M6; OK; TCSF; CD147 antigen; M6 leukocyte activation antigen; OK blood group antigen; basigin; collagenase stimulatory factor; extracellular matrix metalloproteinase inducer
Accession Q28740

Image & description

Fig.1. Rat Basigin (BSG) Standard Curve.

Fig.1. Rat Basigin (BSG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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